Subcutaneous inoculation on the medial wing without blood vessel. According to doses that indicated by bottle label, dilute the vaccine with saline,
dip the diluted vaccine with a chicken pox thorn seed needle, 1 dose inoculation for 20-30 days old chicken; 2 doses inoculation for 30 days; 1 dose inoculation after double dilution for 6-20 days old chicken. There will be slight redness and scab at the inoculation site after inoculation 3-4 days and the scab will be fallen off after 14-21 days. The replacement breeders can be inoculated again 60 days after inoculation.